Journal: International Journal of Molecular Sciences

Article Title: Cellular and Biochemical Characterization of Mesenchymal Stem Cells from Killian Nasal Polyp

doi: 10.3390/ijms232113214

Figure Lengend Snippet: Adipocyte differentiation of KNP-MSCs and HNT-MSCs. ( A ) KNP-MSCs and HNT-MSCs were cultured with a standardized adipogenesis differentiation medium and, after 20 days, stained with Oil Red O to detect lipid droplet amount under 40× magnification of phase contrast microscopy. Scale bar: 100 μm. ( B , C ) ImageJ Fiji software analyses were performed to quantify the percentage of red stained area and the mean of red intensity after 20 days of adipogenic differentiation. ( D , E ) Triglyceride-Glo™Assay was performed to assess triacylglycerols and glycerol levels after 20 days of adipogenic differentiation. ( F – I ) The adipogenic differentiation-related gene expression of PPARγ2, FABP4, Adipo-Q, and LPL were evaluated by RT-qPCR after 10 and 20 days in adipogenic differentiation medium, using the housekeeping gene GAPDH for normalization. Data are displayed as means ± SD from three different experiments (** p < 0.001; *** p < 0.0001).

Article Snippet: To induce adipogenic differentiation, 1 × 10 4 cells/cm2 were seeded in 12-well plates and incubated for 20 days with STEM PRO ® Adipogenesis differentiation medium (cat. No. A1007001 Life Technologies).

Techniques: Cell Culture, Staining, Microscopy, Software, Glo Assay, Expressing, Quantitative RT-PCR

Journal: Frontiers in Veterinary Science

Article Title: Canine adipose tissue-derived MSCs engineered with mRNA to overexpress TSG-6 and enhance the anti-inflammatory effects in canine macrophages

doi: 10.3389/fvets.2023.1134185

Figure Lengend Snippet: Characterization of cAT-MSCs isolated from canine adipose tissue. (A–C) Trilineage potential of cAT-MSCs. Differentiation into adipocyte, osteocyte, and chondrocyte was confirmed via Oil Red O, Alizarin Red, and Alcian Blue staining, respectively. (D–G) Flow cytometry analysis of cell surface markers. cAT-MSCs expressed CD29 and CD90 and lacked CD34 and CD45. (Blue: stained marker, red: unstained negative control).

Article Snippet: To assess their differentiation capacities, cAT-MSCs were cultured using three differentiation media (StemPro Adipogenesis Differentiation, Stem Pro Osteogenesis Differentiation, and StemPro Chondrogenesis Differentiation kits; Gibco).

Techniques: Isolation, Staining, Flow Cytometry, Marker, Negative Control

Journal: Frontiers in Veterinary Science

Article Title: Canine adipose tissue-derived MSCs engineered with mRNA to overexpress TSG-6 and enhance the anti-inflammatory effects in canine macrophages

doi: 10.3389/fvets.2023.1134185

Figure Lengend Snippet: cAT-MSCs expressing GFP with three different UTRs. (A) Sequences of HBA1, HBA2 , and HBB UTR regions. (B) Schematic structure of each template DNA, consisting of a T7 promoter, a 5′-UTR, a GFP coding region, and a 3′-UTR. (C) The highest fluorescence level was observed with HBA1 24 h post-transfection. HBA2 and HBB reached peak fluorescence levels at 12 and 72 h post-transfection, both at lower levels than those of HBA1 . Fluorescence was observed up to 96 h post-transfection. Scale bar, 400 μm ( HBA1 : GFP mRNA with UTR of canine hemoglobin subunit alpha-like 1; HBA2 : GFP mRNA with UTR of canine hemoglobin subunit alpha-like 2; HBB : GFP mRNA with UTR of canine hemoglobin subunit beta-like).

Article Snippet: To assess their differentiation capacities, cAT-MSCs were cultured using three differentiation media (StemPro Adipogenesis Differentiation, Stem Pro Osteogenesis Differentiation, and StemPro Chondrogenesis Differentiation kits; Gibco).

Techniques: Expressing, Fluorescence, Transfection

Journal: Frontiers in Veterinary Science

Article Title: Canine adipose tissue-derived MSCs engineered with mRNA to overexpress TSG-6 and enhance the anti-inflammatory effects in canine macrophages

doi: 10.3389/fvets.2023.1134185

Figure Lengend Snippet: TSG-6 mRNA transfection increased expression of TSG-6 in cAT-MSCs. (A) TSG-6 mRNA level was increased in cAT-MSCs transfected with TSG-6 mRNA (MSC TSG-6 ). (B) TSG-6 protein level was increased in MSCs TSG-6 . (C) Secretion of TSG-6 was also increased in MSCs TSG-6 . Results are shown as the mean ± standard deviation. ** P < 0.01, **** P < 0.0001.

Article Snippet: To assess their differentiation capacities, cAT-MSCs were cultured using three differentiation media (StemPro Adipogenesis Differentiation, Stem Pro Osteogenesis Differentiation, and StemPro Chondrogenesis Differentiation kits; Gibco).

Techniques: Transfection, Expressing, Standard Deviation

Journal: Journal of Clinical Orthopaedics and Trauma

Article Title: Intraarticular injection of allogenic chondroprogenitors for treatment of osteoarthritis in rabbit knee model

doi: 10.1016/j.jcot.2018.07.003

Figure Lengend Snippet: Differentiation studies of Chondroprogenitors. A (control-adipogenic) and C (test-adipogenic) were with stained Oil Red O. B (control-osteogenic) and D (test-osteogenic) were stained with Alizarin Red (mag 20x).

Article Snippet: Passage 2 CPs were subjected to either one of the following differentiation conditions containing Stem Pro adipogenic differentiation medium or Stem Pro osteogenic differentiation medium (Thermo Fischer Scientific).

Techniques: Control, Staining